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KMID : 0043319890120030176
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Archives of Pharmacal Research
1989 Volume.12 No. 3 p.176 ~ p.180
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The Cloning of MLS Antibiotics Inducible Resistance Gene
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Choi EC
Woo KW/Woo JS/Kwak JH/Kim BK
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Abstract
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Four bacterial strains having inducible resistance to erythromycin were isolated from soil samples in Korea and characterized. MLS inducibility was checked in each strain. Cloning of inducible resistance gene(s) has been tried. Four isolates were identified as B. anthracis, Micrococcus luteus, Streptococcus faecium and B. licheniformis, in which erythromycin, oleandomycin, cirramycin and carbomycin acted as resistance inducers respectively. The resistance gene cloned from B. licheniformis 597 strain using pBS 42 vector was found to have a 3.2kb insert.
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